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Expression of ICAM‐1 in implanted primary and metastatic squamous cell carcinomas in rats
Author(s) -
Li Xinwei,
Fujikura Yoshihisa,
Wang YuHsueh,
Sawada Tomoo,
Tokuda Nobuko,
Lovely Rehana Sultana,
Hayatsu Yoshikazu,
Fukumoto Tetsuo,
Shinozaki Fumihiko
Publication year - 1997
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1997.tb00233.x
Subject(s) - immunostaining , pathology , primary tumor , biology , ex vivo , immunohistochemistry , lymph node , cell adhesion molecule , cancer research , metastasis , medicine , cancer , in vivo , immunology , microbiology and biotechnology , genetics
FF6 tumor cells are dervied from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an in vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule‐1 (ICAM‐1) in the implanted primary and metastatic FF6 tumors by immunostaining with a monoclonal antibody (mAb) against ICAM‐1. The implanted primary FF6 cells showed strong expression of ICAM‐1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM‐1. By imiminostaining with rnAb OX6, a number of MHC class II‐positive macro‐phages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM‐1 on tumor cells, and that MHC class II‐positive macrophages may play a role in transplanted tumor growth and metastases.