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Detection of herpes simplex virus type 1 shedding in the oral cavity by polymerase chain reaction and enzyme‐linked immunosorbent assay at the prodromal stage of recrudescent herpes labialis
Author(s) -
Scott D. A.,
Coulter W. A.,
Biagioni P. A.,
O'Neill H.,
Lamey P.J.
Publication year - 1997
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1997.tb00220.x
Subject(s) - herpes simplex virus , polymerase chain reaction , virology , alphaherpesvirinae , biotinylation , virus , herpesviridae , biology , hsl and hsv , amplicon , digoxigenin , viral shedding , microbiology and biotechnology , real time polymerase chain reaction , agarose gel electrophoresis , simplexvirus , viral disease , dna , in situ hybridization , gene expression , gene , biochemistry , genetics
Recrudescent herpes labialis (RHL) is a disease caused by herpes simplex virus (HSV). predominantly type 1 (HSV‐1). We have monitored HSV‐1 shedding in the oral cavity by polymerase chain reaction (PCR) and enzyme‐linked immunosorbent assays (ELISA) using digoxigenin‐labeled primers designed to amplify a 278 bp segment of the HSV‐1 UL 42 region. Prodromal RHL was confirmed by thermographic imaging in 22 patients. Infectious virus was not detected using tissue culture for virus isolation (0/22). Using PCR and agarose gel electrophoresis. We could detect HSV‐1 DNA in 8/22 patients. Using a biotinylated‐probe internal to the predicted sequence of the PCR product. HSV‐1 DNA was detected in 10/22 of the patients by ELISA. We conclude that HSV‐1 DNA is shed into the oral cavity of patients presenting with sub‐clinical RHL and that the PCR‐EL1SA technique represents a more sensitive method to monitor HSV‐1 shedding than conventional tissue culturing or PCR‐electrophoresis alone.