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Mast cell‐derived tryptase in odontogenic cysts
Author(s) -
Teronen O.,
Hietanen J.,
Lindqvist C.,
Salo T.,
Sorsa T.,
Eklund K. K.,
Sommerhoff C. P.,
Ylipaavalniemi P.,
Konttinen Y. T.
Publication year - 1996
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1996.tb00282.x
Subject(s) - tryptase , odontogenic , mast cell , pathology , medicine , immunology
Inflammatory and developmental cysts of the jaws are relatively common bone destructive lesions in the human maxillofacial skeleton but their pathogenesis is still poorly understood. In this study the role of mast cells (MC), and mast cell tryptase in particular, was evaluated in the pathophysiology of bone resorption and jaw cyst formation in different types of cysts. The distribution of MC and the amount of tryptase in histological tissue sections were determined by immuno‐histochemistry using monoclonal antihuman tryptase antibodies and the results were quantitated by using an image analyzing system. The amount of tryptase was further studied by Western‐blotting and measurement of trypsin‐like activity from the neutral salt extracts obtained from different types of jaw cysts. In contrast to control tissue, high trypsin‐like activities and abundant immunoreactive tryptase were observed in the extracts of all types of cysts studied (radicular, dentigerous and keratocyst). In tissue sections the highest amount of tryptase‐positive staining was observed in radicular cysts (mean 6.2% of reference area) and the lowest amount in keratocysls (mean 2.1% of reference area. >O.O1). MC were found to be located in inflammatory cell‐rich tissue areas and just beneath the cyst epithelium. Importantly, MC located at the border of bone were observed to be degranulated. indicating high activity of MC and release of tryptase at the regions of early bone destruction, Based on previous findings addressing the role of mast eel! tryptase in proteolytic cascades, and the known association of MC with osteoporosis, we suggest that mast cells and mast cell tryptase may contribute significantly to jaw cyst tissue remodelling during growth of a cyst, and to the destruction of the surrounding bone, resulting in jaw cyst expansion.