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Immunohistochemical localization of fibroblast growth factor‐1 (FGF‐l)and FGF‐2 in cultured human ameloblastoma epithelial cells and ameloblastoma tissues
Author(s) -
Myoken Y.,
Myoken Y.,
Okamoto T.,
Sato J. D.,
Takada K.
Publication year - 1995
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1995.tb01206.x
Subject(s) - fibroblast growth factor , epithelium , fibroblast , biology , ameloblastoma , basic fibroblast growth factor , immunoperoxidase , basement membrane , immunohistochemistry , pathology , microbiology and biotechnology , growth factor , cell culture , immunology , anatomy , medicine , biochemistry , monoclonal antibody , antibody , maxilla , genetics , receptor
Fibroblast growth factor‐1 (FGF‐1) and FGF‐2 are mitogenic polypeptides that may contribute to neoplastic cell proliferation. In the present study, we established a serum‐free culture system for ameloblastoma cells and demonstrated that the addition of FGF‐1 and FGF‐2 enhanced cell growth in a dose‐dependent manner. Immunoperoxidase staining of cultured cells demonstrated strong expression of FGF‐1 and FGF‐2. In tissue specimens, FGF‐1 was localized in epithelial cell components of ameloblastomas, whereas FGF‐2 was mainly found in the basement membranes with only moderate staining in epithelium. These data suggest that both FGF‐1 and FGF‐2 may contribute to the growth and development of ameloblastomas.