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Expression of p53 protein in oral squamous cell carcinomas and adjacent non‐tumorous mucosa of the floor of the mouth: an archival immunohistochemical study using wet autoclave pretreatment for antigen retrieval
Author(s) -
Piffkó József,
Bánkfalvi Àgnes,
Öfner Dietmar,
Berens Axel,
Tkotz Thomas,
Joos Ulrich,
Böcker Werner,
Schmid Kurt Werner
Publication year - 1995
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1995.tb01196.x
Subject(s) - immunohistochemistry , pathology , floor of mouth , oral mucosa , antigen retrieval , antigen , antibody , medicine , immunology , tongue
Routinely formalin‐fixed and paraffin‐embedded material of 22 squamous cell carcinomas of the floor of the mouth (T2NoMo, Ro), together with adjacent dysplastic or normal mucosa, were immunohistochemically investigated using a panel of four anti‐p53 antibodies (CM1, PAbl801, DO7, PAb240) subsequent to wet autoclave pretreatment for antigen retrieval. p53 immunoreactivity was detected in 9/22 (40%) carcinomas with PAbl801 and DO7 antibodies, and in 8/22 cases using CM1 and PAb 240. p53‐positive tumour cells accumulated predominantly at the invasive front of the carcinomas. A focal or scattered p53 immunoreactivity was observed in the adjacent normal and/or dysplastic mucosa in 17/22 (77%) cases using both CM1 and PAbl801 antibodies, in 10/22 with DO7, and in 8/22 with PAb240. This study has demonstrated examples of different p53 immunophenotypes in the non‐tumorous and neoplastic oral mucosa of the same patient without significant correlation to the clinicopathological parameters studied.