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Demonstration of c‐ erb B‐2 oncogene overexpression in salivary gland neoplasms by in situ hybridization
Author(s) -
Jordan Richard,
Dardick Irv,
Lui Eugene,
Birek Catalena
Publication year - 1994
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1994.tb01118.x
Subject(s) - salivary gland , in situ hybridization , acinic cell carcinoma , pathology , biology , oncogene , cell , messenger rna , gene expression , mucoepidermoid carcinoma , microbiology and biotechnology , gene , medicine , cell cycle , biochemistry
The level of c‐erbB‐2 cellular mRNA in 18 salivary gland tumours and in 7 normal salivary glands was determined by in situ hybridization using [ 35 S] labelled RNA probes. Computer assisted quantitation of the autoradiographic signal indicated a significantly higher c‐ erb B‐2 expression in the tumour group (22.64 grains per cell ±3.79; 95% CI) as compared to the non‐neoplastic salivary gland tissue (4.11 ±0.90; 95% CI). The c‐erbB‐2 expression as measured by grain counts per cell for the pleomorphic adenomas (16.29± 1.87; 95% CI), mucoepidermoid carcinomas (31.52 ±0.08; 95% CI) and the acinic cell carcinomas (44.24± 17.11; 95% CI) were significantly greater than the expression for the normal group. The acinic cell carcinomas exhibited the greatest level of expression. As observed at the individual cell level, the autoradiographic signal was distributed uniformly in the neoplastic tissues, regardless of the cell type. This study confirms the hypothesis that the c‐erb B‐2 oncogene is overexpressed at the mRNA level in salivary gland tumours.