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IL‐4‐ and IL‐6‐producing cells in human periodontal disease tissue
Author(s) -
Yamazaki Kazuhisa,
Nakajima Takako,
Gemmell Erica,
Polak Boris,
Seymour Gregory J.,
Hara Kohji
Publication year - 1994
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1994.tb00074.x
Subject(s) - gingivitis , connective tissue , in situ hybridization , immunohistochemistry , epithelium , pathology , periodontitis , biology , messenger rna , medicine , dentistry , gene , biochemistry
IL‐4‐ and IL‐6‐producing cells in human periodontal disease tissues were investigated using immunohistochemical and in situ hybridization techniques. Immunohistochemical analysis demonstrated the presence of IL‐4‐producing cells within the CD45RO + subset and the percentage of IL‐4+ cells was significantly higher in periodontal lesions than in gingivitis tissues (p<0.01). The percentage of IL‐6‐producing memory cells was higher in periodontal lesions compared with gingivitis tissues, although it was not statistically significant (p<0.05). A reverse tendency in IL‐4‐ and IL‐6‐positive cells was observed in a few individual cases. No IL‐4 mRNA could be detected using the in situ hybridization technique. However, high levels of IL‐6 mRNA were present in clinically healthy tissues, with a further increase in both epithelium and connective tissues affected by gingivitis, although only the former was significant (p< 0.025). There was a significant decrease in IL‐6 mRNA in both the connective tissue (p<0.025) and epithelium (p<0.01) in periodontitis tissues compared with levels in gingivitis tissues. However, the levels of IL‐6 mRNA in periodontal tissues were high compared with those of IL‐1 mRNA, which was used in this study as a positive control. These results suggest that Th2–type cells may accumulate in periodontal lesions.