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Detection of Epstein‐Barr virus and human papillomavirus type 16 DNA in hairy leukoplakia by in situ hybridisation and the polymerase chain reaction
Author(s) -
Felix D. H.,
Jalal H.,
Cubie H. A.,
Southam J. C.,
Wray D.,
Maitland N. J.,
Felix D. H.
Publication year - 1993
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1993.tb01071.x
Subject(s) - polymerase chain reaction , virology , biology , digoxigenin , in situ hybridization , epstein–barr virus , in situ , virus , oligonucleotide , microbiology and biotechnology , hybridization probe , papillomaviridae , dna , gene , chemistry , genetics , cancer , cervical cancer , organic chemistry , gene expression
Demonstration of Epstein‐Barr virus (EBV) is considered desirable for the accurate diagnosis of hairy leukoplakia (HL). Previous studies have reported possible associations with human papillomavirus (HPV) infection although this is not a universal finding. Presence of EBV and HPV 16 was examined in biopsy specimens from 18 cases of HL and ten control specimens by in situ hybridisation using digoxigenin‐labellcd synthetic oligonucleotide probes and by the polymerase chain reaction (PCR). The presence of EBV was demonstrated in 12 cases by both techniques. Of the remaining six cases EBV could be detected in three by in situ hybridisation but not by PCR; EBV was not detected by either method in a further three cases. All samples were negative for HPV 16 by both techniques under conditions of high stringency, although when stringency of in situ hybridisation was reduced, four samples appeared to harbour HPV DNA sequences. This study provides further evidence to support the role of EBV in the pathogenesis of HL and suggests that HPV 16 is not regularly encountered.