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Human papillomaviruses in normal oral mucosa: a comparison of methods for sample collection
Author(s) -
Lawton Guy,
Thomas Steve,
Schonrock Joy,
Monsour Frank,
Frazer Ian
Publication year - 1992
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1992.tb01008.x
Subject(s) - oral mucosa , genotype , medicine , buccal swab , sex organ , buccal mucosa , population , human papillomavirus , pathology , biology , oral cavity , dentistry , gene , genetics , environmental health
The prevalence of six genital genotypes of HPV was assessed in the clinically normal oral mucosa of an adult Caucasian population, and three methods of sample collection compared. HPV DNA was detected in the mouth of 60% of 60 subjects. HPV 16 was the most prevalent genotype, and positive samples were found most frequently in men over 50. A 3% sucrose mouthwash produced more positive results (51%) than mucosal scrapes of three separate sites (45%) or buccal mucosal biopsies (12%). There was no association of a positive result for HPV DNA with any particular mucosal site. A mouthwash was the preferred single screening method for epidemiologic studies of HPV DNA in the mouth, but the greatest yield of positive samples was obtained if multiple sampling techniques were employed.