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Transamidase and collagenase activity in healthy and diseased human gingival tissues
Author(s) -
Robinson P. J.,
Siew C.,
Gruninger S. E.,
Chang S.B.,
Turner D. W.,
Harper D. S.
Publication year - 1992
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1992.tb00977.x
Subject(s) - collagenase , enzyme , chemistry , tris , biochemistry , centrifugation , lysine , microbiology and biotechnology , biology , amino acid
Transamidases are a class of calcium–dependent mammalian enzymes which crosslink proteins by catalyzing the formation of (γ–glutamyl)–ε–lysine bonds. It is possible that these enzymes play an important anabolic role in tissue healing. This study was to quantitate transamidase activity in human gingival tissue and examine the relation between transamidase activity and degree of inflammation. Forty–four out of a total 120 collected human gingival specimens from healthy and diseased patients were selected based on histometric and microbiologic criteria. Specimens were minced and homogenized in 10 mM CaCl 2 and then extracted for 30 min, in 50 mM tris–HCl buffer (p H 7.5) containing 100 mM CaCl 2 . Following low speed centrifugation at 4 C, the supernatant solution was assayed for both transamidase and collagenase activities by radioactive amine incorporation, and digestion of tritiated collagen, respectively. Appreciable levels of transamidase and collagenase activities in healthy gingivae were found. These enzyme activities were significantly elevated in the diseased and healing tissues. Unlike other transamidases, calcium was required in the enzyme extraction process.