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Epstein‐Barr virus receptors but not viral DMA are present in normal and malignant oral epithelium
Author(s) -
Talacko A. A.,
Teo C. G.,
Griffin B. E.,
Johnson N. W.
Publication year - 1991
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1991.tb00882.x
Subject(s) - epithelium , pathology , virus , biology , epstein–barr virus , oral mucosa , stratified squamous epithelium , in situ hybridization , staining , monoclonal antibody , carcinogenesis , antibody , microbiology and biotechnology , cancer , virology , immunology , medicine , messenger rna , biochemistry , genetics , gene
The presence and distribution of Epstein‐Barr Virus receptors (EBVR's) on a range of normal ( n = 18), dysplastic ( n = 10) and malignant ( n = 20) oral mucosa were studied by immunocytochemical methods using the monoclonal antibodies (MAb's) HB5 and B2. EBVR's were demonstrated as membrane staining of the spinous layers of normal non‐ and parakeratinized epithelium, indicating that EBVR's are differentiation‐linked. This distribution was retained in dysplastic epithelium. Tissue from oral squamous cell carcinomas (SCC's) showed variable reactivity of only a few cells scattered randomly within the samples. Furthermore, a sensitive in situ hybridization (ISH) technique was used to determine if Epstein‐Barr virus (EBV) was present in normal ( n = 15) and oral squamous cell carcinoma tissues ( n = 20). No EBV DNA was demonstrated within either normal or malignant epithelium, suggesting that the virus does not persist in normal oral stratified squamous epithelium nor is there any evidence for a role in oral carcinogenesis.