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Immunohistochemical localization of Leu‐M 1 carbohydrate antigen in human oral squamous cell carcinoma
Author(s) -
Teng Y.T.,
Nadimi H.,
Toto P.O.
Publication year - 1989
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1989.tb01351.x
Subject(s) - antigen , pathology , epithelium , stratum spinosum , immunohistochemistry , biology , cell , oral lichen planus , microbiology and biotechnology , immunology , medicine , stratum corneum , biochemistry
Twenty‐six biopsy specimens of oral squamous cell carcinomas were examined by the avidin biotin peroxidase complex (ABC) method for the presence of an epithelial cell membrane bound lacto‐N‐fucopentaose III, known also as Leu‐M 1 or Le x antigen. In normal oral epithelium, Leu‐M 1 antigen was expressed on keratinizing epithelia in the stratum spinosum. In well‐differentiated carcinomas the antigen was found on the cell membrane of nucleate cells in infiltrating epithelial islands. Such pattern in moderately welland in poorly differentiated carcinomas was minimally expressed and was associated with flattened squamous cells or otherwise recorded negative. Leu‐M, antigen immunoreactivity in normal oral epithelia and in carcinomas was comparable to that of blood group H‐2 chain that were examined. It was concluded that the intensity of the reaction parallels the magnitude of differentiation of epithelia. Leu‐M 1 antigen can serve as a marker of differentiation in oral squamous epithelium.