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Explants of human oral epithelium exposed to viruses and cancer chemotherapeutics
Author(s) -
Ebbesen P.,
Petersen P.M.,
Jepsen A.,
NorskovLauritsen N.,
Nielsen C. M.,
Philipsen H. P.,
ArenholtBindslev D.,
Nara P.
Publication year - 1989
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1989.tb01347.x
Subject(s) - virus , virology , herpes simplex virus , biology , oncolytic virus , measles virus , viral culture , epithelium , measles , vaccination , genetics
Cultures of proliferating epithelial cells were established from explants of normal human oral epithelium from healthy young volunteers. The epithelial cells were found permissive for herpes simplex virus type 1 and type 2, coxsackie virus A‐4 and A‐16, adenovirus type 5, measles vaccine, rubella and influenza type A virus‐. Medium from DEAE‐pretreated epithelial cultures infected with two subtypes of human immunodeficiency virus‐1 showed an increasing content of virusprotein with time by antigen ELISA testing. In contrast there was no evidence of infection with coxsackie virus type B‐2, cytomegalovirus, Epstein‐Barr virus and varicella zoster virus. Treatment of the epithelial cells with a non‐cytotoxic dose of cancer chemotherapeutic prior to or after infection with coxsackie virus A‐4 or herpes simplex virus type 1 influenced the virus production dependent on both compound, mode of application, and virus. Adriamycin (doxorubicin) in low dose was found to stimulate the production of the two viruses.