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Establishment and characterization of an epithelial cell line from the rat submandibular gland
Author(s) -
Brown Ashley M.,
Rusnock Eileen J.,
Sciubba James J.,
Baum Bruce J.
Publication year - 1989
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1989.tb00764.x
Subject(s) - submandibular gland , antiserum , keratin , cytoplasm , electron microscope , epithelium , biology , salivary gland , pathology , ductal cells , parotid gland , acinus , cytoskeleton , cell culture , microbiology and biotechnology , antigen , chemistry , cell , immunohistochemistry , endocrinology , immunology , medicine , biochemistry , pancreas , physics , genetics , optics
An epithelial cell line, RSMTx, has been established from the submandibular gland of weanling Fisher 344 rats by treatment of explanted tissue clumps with 3‐methylcholanthrene. These cells exhibit a polygonal shape on light microscopy and a polar appearance, with desmosomes, terminal bar‐like structures, surface microvilli and cytoplasmic interdigitations, when examined by electron microscopy. The cells react positively with an antiserum to cytoskeletal keratin, and a commercial monoclonal antibody to an “epithelial membrane antigen.” An antiserum, prepared against early passage cells in hamsters, reacts primarily with ductal elements in tissue sections of submandibular gland, as does an antiserum prepared in mice with late passage cells. The cells are easily passaged and have been maintained for more than two years in continuous culture.