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An immunocytochemical study of the inflammatory cell infiltrate and epithelial expression of HLA‐DR in odontogenic cysts
Author(s) -
Matthews J. B.,
Browne R. M.
Publication year - 1987
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1987.tb01476.x
Subject(s) - immunoperoxidase , pathology , radicular cyst , cyst , biology , epithelium , cd8 , odontogenic cyst , macrophage , antigen , immunology , medicine , monoclonal antibody , antibody , in vitro , biochemistry
The presence and distribution of lymphocytes, Langerhans cells, HLA‐DR' infiltrating cells and the expression of HLA‐DR by lining epithelium was investigated in the walls of odonlogenic cysts using an indirect immunoperoxidase method on acetone‐fixed cryostat sections. The 23 cysts studied consisted of 14 dental (radicular) cysts, 5 keratocysts, 2 dentigerous cysts, 1 surgical ciliated cyst and 1 incisive canal cyst. The cell populations detected in the walls of all cysts were similar and consisted of HLA‐DR+ macrophage‐type cells and a mixture of T and B lymphocytes. Analysis of the T cell subsets revealed that in all cases the CD4 + , T h/i subset predominated over the CD8 + , T s/c subset. 18/22 cyst linings contained cells expressing a Langerhans cell phenotype (CD1 + ). Cytoplasmic epithelial expression of HLA‐DR was detected in 7/22 specimens. Neither the presence of HLA‐DR + epithelial cells nor LC were restricted to a given type of cyst. These findings indicate the occurrence of similar cellular processes irrespective of the proposed developmental or inflammatory aetiology of these lesions.