Premium
Nuclear DNA analysis of oral hyperplasia and dysplasia using image cytometry
Author(s) -
AbdelSalam M.,
Mayall B. H.,
Hansen L. S.,
Chew K. L.,
Greenspan J. S.
Publication year - 1987
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1987.tb00713.x
Subject(s) - dysplasia , stain , pathology , hyperplasia , linear discriminant analysis , medicine , mathematics , staining , statistics
We investigated the value of image analysis in discriminating among oral white lesions with hyperplasia without dysplasia and oral white or white‐and‐red lesions with moderate or severe dysplasia. Normal oral epithelial tissue was used as a control. Image analysis was applied to 5‐μm formalin‐fixed sections stained with the azure A‐Feulgen reaction for nuclear DNA. For 150–200 cells from each section, 5 nuclear variables were assessed: area, form factor, total stain, average stain and ellipticity. For each variable, 2 measurements were obtained, the mean and the interquartile range, and were used for stepwise discriminant analysis. Using this test, a model of 3 measurements with the most discriminating power was developed. When the jackknife classification test was applied to this model, we could discriminate with 81% accuracy between the 4 groups of tissue studied.