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Angiogenic activity of malignant oral epithelial cells: a preliminary study
Author(s) -
Herwagen D.,
ArenholtBindslev D.,
Dabelsteen E.
Publication year - 1987
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1987.tb00685.x
Subject(s) - epithelium , angiogenesis , pathology , cell culture , biology , epidermis (zoology) , oral mucosa , cell , neovascularization , immortalised cell line , cancer research , medicine , anatomy , genetics
We investigated the angiogenetic capacity of normal and malignant oral epithelial cells by intradermal injection of cells into nude mice. Cells investigated: 1) a tumorigenic cell line from rat palate; 2) a non‐tumorigenic epithelial cell line from rat palate; 3) cells from second passage of rat palate epithelium explant cultures; 4) cells from epidermis of neonatal mice. A 0.1 ml suspension containing 10 5 epithelial cells was injected into the flank region. Mice were killed 8 days later to evaluate angiogenesis. Under a dissection microscope, the number of vessels in the skin adjacent to the injection site was counted. The tumorigenic cell line demonstrated the strongest angiogenetic capacity and the non‐tumorigenic cell line was more angiogenic than any of the other non‐tumorigenic cell types. The greater angiogenic activity of neoplastic oral epithelial cells may be a useful feature in the evaluation of oral malignant development.