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S‐100 immunolabelling of Langerhans cells in oral epithelium
Author(s) -
Charbit Y.,
Monteil R. A.,
Hitzig C.,
Sanget P.,
Benaiche N.,
Jasmin J. R.
Publication year - 1986
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1986.tb00650.x
Subject(s) - epithelium , staining , immunohistochemistry , antiserum , pathology , langerhans cell , biology , antigen , microbiology and biotechnology , immunology , chemistry , medicine
Langerhans cells (LCs) are dendritic cells usually present in the suprahasal regions of squamous epithelial remarkable by the presence of Fc IgG and C 3 receptors, Ia/Or, T‐6 and T‐4 antigens on their membranes. Conventional and immunohistochemical techniques for identification of LCs require special fixation procedures and are incompatible with paraffin embedding. A method for staining LCs, in routine fixed and paraffin‐embedded material, based on the use of antiserum against S‐100 proteins is presented. The problem arising from the simultaneous staining of melanocytes is discussed. S‐100 positive dendritic cells should be considered as LCs when fulfilling 3 criteria: 1) located in the suprabasal layers of the epithelium: 2) the whole cell body being visible: 3) with at least one dendritic process.