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Immunohistochemical localization of amylase in sialoadenitis and salivary gland tumors
Author(s) -
Sumitomo S.,
Kumasa S.,
Tatemoto Y.,
Ookusa Y.,
Mori M.
Publication year - 1986
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1986.tb00645.x
Subject(s) - pathology , serous fluid , immunohistochemistry , staining , salivary gland , ductal cells , amylase , biology , lumen (anatomy) , submandibular gland , adenolymphoma , medicine , enzyme , microbiology and biotechnology , biochemistry
Immunohistochemical demonstration of α‐amylase has been made in sialoadenitis‐involved tissue and salivary gland tumors, as well as in normal salivary glands. Immunoreactive α‐amylase with trypsin pretreatment was confined to irregularly staining serous acinar cells in the parotid and submandibular glands, and to demilunes in subligual glands. In obstructivce adenitis, staining was irregular from high to negative in acini in early or intermediate stages. Dilated ductal segments contained cells positive for α‐amylase in the early stage following obstruction. Pleomorphic adenomas were usually negative for α‐amylase but in rare cases tumor epithelia stained variably positive; i.e., staining occurred throughout the cytoplasm or at the periphery or apical part of the tumor cells. Luminal cavities of tubular and duct‐like structures contained α‐amylase‐positive material. Epithelia in Warthin's tumor were also negative in general; however, scattered single or grouped tumor cells containing α‐amylase were found. Mucoepidermoid tumors were also negative, though slightly positive cells were found intermingled among the negative squamous and mucous tumor cells. Cystic lesions in mucoepidermoid tumor were sometimes positive in the wall cells together with material secreted into the lumen.