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Modulation of Class II (DR and DQ) antigen expression on gingival Langerhans cells in vitro by gamma interferon and prostaglandin E2
Author(s) -
Walsh L. J.,
Seymour G.J.,
Powell R. N.
Publication year - 1986
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1986.tb00638.x
Subject(s) - in vitro , prostaglandin e2 , antigen , interferon gamma , prostaglandin e , in vivo , microbiology and biotechnology , cell culture , interferon , hla dr , biology , chemistry , immunology , endocrinology , biochemistry , genetics
The effect of human and murine gamma interferon (IFN‐γ) on Class II (DR and DQ) expression by human gingival Langerhans cells (LC) was examined using an organ culture system. Human and murine IFN‐γ increased the expression of both DR and DQ antigens during a 72‐h period. Maximal levels of DR expression (100% of T6 + cells) were produced within 6 h of IFN‐γ treatment and were maintained during culture. DQ expression was maximal (100% of T6 + cells) at 18 h and was more labile than DR. The effect of IFN‐γ on DR and DQ was abrogated by prostaglandin E2 (PGE) while indomethacin, and inhibitor of PGE synthesis, mimicked the effect of IFN‐γ on DR and DQ expression. Gingival keratinocytes maintained their expression of DR for 72 h in the presence of γ‐IFN, but did not express DQ under any of the culture conditions used in this study. These results demonstrate that γ‐IFN induces differential expression of Class II antigens in the oral mucosa in vitro , and suggest that γ‐IFN and PGE may be involved in the control of DR and DQ antigen expression in vivo.