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Detection of herpes simplex virus proteins in cultured cells by monoclonal antibodies and the avidin‐biotin‐immunoperoxidase complex method
Author(s) -
Hwang C. B. C.,
Greenspan J. S.,
Shillitoe E. J.
Publication year - 1986
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1986.tb00602.x
Subject(s) - immunoperoxidase , monoclonal antibody , avidin , herpes simplex virus , biotin , virology , biology , antibody , microbiology and biotechnology , monoclonal , simplexvirus , virus , herpesviridae , viral disease , immunology , biochemistry
The distribution of 5 proteins of herpes simplex virus Type 1 was observed in cells that had been infected for various periods. The cells were stained with monoclonal antibodies to 1CP4, ICP5, ICP6, ICP8, and gB, using the avidin‐biotin‐peroxidase complex (ABC) method. Each protein had a characteristic pattern of time of appearance and translocation by which it could be distinguished from the others. The sensitivity of the ABC technique, its ease of use, and the permanence of the preparations make this method well suited for the study of viral proteins.