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Histologic study of the inhibition of bone resorption in organ cultures by myoinositol‐2‐monophosphate
Author(s) -
Gomes B. C.,
Kaufman H. W.,
Archard H. O.,
Cangemi C.,
Guerra A.
Publication year - 1986
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1986.tb00564.x
Subject(s) - resorption , parathyroid hormone , bone resorption , endocrinology , medicine , fetus , organ culture , chemistry , phosphate , hormone , calcium , biology , biochemistry , in vitro , pregnancy , genetics
This study examines chemically and histologically the relative abilities of inositiol monophosphate (IP1), inorganic phosphate (Pi), ethane‐1‐hydroxy‐1, 1‐diphosphonate (EHDP) and dichloromethylene disphosphonate (Cl 2 MDP) to inhibit parathyroid hormone (PTH) ‐ induced resorption of fetal rat long bones in organ culture. Pregnant rats injected with 45 Ca on the 18th day of gestation were killed the next day and their fetuses removed. Half of each pair of dissected long bones was incubated in a chemically defined control medium while the contralateral half was incubated in medium containing PTH or PTH plus the compound to be tested. 45 Ca released into the medium was indicative of the amount of bone resorption. Bones were then processed histologically and examined microscopically. All compounds inhibited resorption to some extent with IP1 and Pi being less effective than EHDP or Cl 2 MDP at comparable phosphate concentrations. However, the disphosphonates damaged osteoclasts whereas IP1 and Pi did not. This suggests that IP1 may inhibit resorption by a different mechanism perhaps related only to prevention of crystal dissolution.