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Effect of inflammation upon human gingival cyclic AMP metabolism
Author(s) -
Fink A. S.,
Egnor R. W.,
Kazandjian G.,
La Morte G.,
Klonsky K.
Publication year - 1985
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1985.tb00502.x
Subject(s) - cyclase , adenylate kinase , phosphodiesterase , inflammation , basal (medicine) , endocrinology , medicine , gingival inflammation , chemistry , stimulation , enzyme , biochemistry , periodontal disease , insulin
The effect of the increasing degree of human gingival inflammation on adenylate cyclase (basal, fluoride stimulated) and low Km and high Km cAMP phosphodiesterase activities were evaluated in separate studies. Human gingival biopsies were classified by the Löe Bleeding Index as (1) mildly, (2) moderately, and (3) markedly inflamed. Basal and F stimulated adenylate cyclase (cAMP synthesis) activities were found to be unaltered by the increasing degree of inflammation when the data were expressed on either a mg wet wt, or mg protein basis. A significant loss of F stimulated adenylate cyclase (mg protein) activity was observed in the moderately inflamed group when the data were compared with either the mildly or markedly inflamed groups of tissue. The low Km, and high Km cAMP phosphodiesterase activities (cAMP degradation) were found to he unaffected by gingival inflammation. This suggests that neither cAMP synthesis, nor degradation are stimulated in human gingiva by inflammation.