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A simplified method for culture of oral epithelial cells
Author(s) -
Schuster G. S.,
Singh B. B.,
Welter D. A.,
Erbland J. F.
Publication year - 1985
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1985.tb00501.x
Subject(s) - epithelium , collagenase , oral mucosa , pathology , ultrastructure , microbiology and biotechnology , biology , cell culture , tissue culture , chemistry , in vitro , anatomy , medicine , biochemistry , genetics , enzyme
In order to facilitate studies on oral mucosa a simplified method for the culture of oral epithelial cells from adult hamsters was developed. Cheek pouches were excised and epithelial cells isolated by collagenase digestion. These were grown in CM‐V medium containing spermine in order to inhibit overgrowth of the epithelial cells by fibroblasts. The epithelial cells were subcultured by routine tissue culture procedures. The cells isolated were examined by light microscopy and scanning and transmission electron microscopy. Morphologically the cells were typical of epithelial cells. Ultrastructural examination showed structures typical of epithelia including filaments, keratohyalin granules and desmosomal junctions. The culture system provides epithelial cells that can be used for a variety of biochemical and morphological studies.