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Modulation of HLA‐DR antigens in the gingival epithelium in vitro by heat‐killed Fusobacterium nucleatum and E. coli lipopolysaccharide
Author(s) -
Walsh L. J.,
Seymour G. J.,
Bird P. S.,
Powell R. N.
Publication year - 1985
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1985.tb00473.x
Subject(s) - fusobacterium nucleatum , antigen , in vitro , epithelium , lipopolysaccharide , monoclonal antibody , microbiology and biotechnology , biology , cytotoxic t cell , mucous membrane , chemistry , immunology , antibody , porphyromonas gingivalis , bacteria , biochemistry , genetics
The in vitro influence of the periodontopathic organism Fusobacierium nucleatum (FN) on gingival tissue was examined using an organ culture system. Treatment of gingival explains obtained from periodontally diseased sites with suspensions of FN, stimulated the expression of HLA‐DR antigens by Langerhans cells (LC) in a dose‐dependent fashion, and produced a maintenance of the LC markers Td and ATPase. Similar effects were seen when E. coli lipopolysaceharide (LPS) was substituted for suspensions of FN. With both FN and LPS the expression of HLA‐DR by gingival keratinocytes was maintained throughout the 72‐h culture period, de‐spite the cytotoxic effects of these agents. Using a variety of immunohistological techniques and a monoclonal antibody specific for the strain of FN used, it was possible to demonstrate the uptake of FN antigens by LC within the gingival epithelium.