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Loss of Langerhans cells from gingival tissue maintained in organ culture
Author(s) -
Walsh L. J.,
Powell R. N.,
Seymour G. J.,
Newcomb G. M.
Publication year - 1984
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1984.tb01462.x
Subject(s) - organ culture , langerhans cell , antigen , epithelium , incubation , human leukocyte antigen , population , biology , immunology , tissue culture , pathology , microbiology and biotechnology , medicine , biochemistry , in vitro , environmental health
Changes occurring in a population of Langerhans cells (LC) were investigated using human gingival epithelium maintained in organ culture. The enzyme adenosine 5′‐triphosphatase together with the surface antigens HLA‐DR and T6 were used to identify and quqntitate these cells. An exponential fall in the expression of each of these markers was seen to occur with increasing incubation lime, suggesting that a true loss of these cells is in fact occurring. HLA‐DR antigen is shed from LC at a rate significantly greater than thai al which LC are lost. A mathematical model which simulates the behaviour of LC within organ culture is presented, and several implications and possible explanations proposed.