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Immunohistochemical demonstration of plasma proteins in squamous epithelium of formalin‐fixed, paraffin‐embedded oral mucosa
Author(s) -
Reibel J.
Publication year - 1984
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1984.tb01403.x
Subject(s) - epithelium , pathology , keratin , immunohistochemistry , connective tissue , oral mucosa , immunofluorescence , biology , chemistry , antibody , medicine , immunology
The distribution of immunoglobulins and fibrinogen in normal and inflamed oral mucosa was examined by direct immunofluorescence using enzyme‐treated sections of formalin‐fixed, paraffin‐embedded tissue. Plasma proteins were found inter‐ and intracellularly in the surface epithelium in most of the normal and all of the inflamed mucosal specimens. This diffusion of plasma proteins into the surface epithelium was shown to contribute to the appearance of edematous epithelial cells and eosinophilic bodies (“keratin pools”). The results clearly demonstrate that oral epithelium is not to be regarded as a covering membrane isolated from the underlying connective tissue. Furthermore, the study confirms that immunohistochemical studies can be performed on enzyme‐pretreated sections of routinely fixed and embedded tissue although it is emphasized that the method is not of universal applicability.