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Establishment of a human cancer cell line from carcinoma of the gingiva
Author(s) -
Koike Masao,
Komori Akira
Publication year - 1982
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1982.tb00151.x
Subject(s) - pathology , keratin , biology , ultrastructure , cell culture , population , carcinoma , karyotype , cell , histology , epithelium , basal cell , chromosome , anatomy , medicine , genetics , environmental health , gene
A new human epithelial cell line (MK—1) has been established from a well differentiated squamous cell carcinoma of the gingiva. It has been subcultured 90 times during the past 23 months. The population doubling time of the MK—1 cell line was 28 h. A colony forming ability with 18% plating efficiency was shown. Chromosomal analysis revealed an aneuploid human male karyotype with 22 marker chromosomes. The modal chromosome number was 75. Scanning electron micrographs of MK—1 cells showed numerous microvilli and intercellular bridges. Transmission electron micrographs of MK—1 cells showed epithelial characteristics with desmosomes, tonofilaments and membrane‐coating granules. Hetero‐transplantation of cultured MK—1 cells produced tumors, the histology of which was similar to that of original ones. The ultrastructure of the tumors showed the presence of abundant tonofibrils and keratohyalin granules. This MK—1 cell line is a model useful for the study of keratinization of squamous cell carcinomas.