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Autoradiographic demonstration of unscheduled DNA synthesis in oral tissues treated with chemical carcinogens in short‐term organ culture
Author(s) -
Ide Fumio,
Ishikawa Takatoshi,
Takayama Shozo,
Umemura Shinichiro
Publication year - 1981
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1981.tb01256.x
Subject(s) - carcinogen , carcinogenesis , organ culture , hamster , in vivo , 4 nitroquinoline 1 oxide , oral mucosa , biology , dna synthesis , salivary gland , pathology , dna , microbiology and biotechnology , chemistry , in vitro , anatomy , biochemistry , cancer , medicine , genetics
A system in which oral tissues of inbred F344 adult rats and Syrian golden hamster embryos were used in combination with autoradiography was developed for measurement of unscheduled DNA synthesis (UDS). For this, oral mucosa, submandibular gland, tooth germ and mandible in short‐term organ cultures were treated with 4‐nitroquinoline 1‐oxide or N‐methyl‐N‐nitrosourea plus (methyl‐ 3 H)thymidine. Significant numbers of silver grains, indicating UDS, were detected over the nuclei of cells of all these tissues except rat salivary gland after treatment with carcinogens. This autoradiographic method is suitable for detection of UDS in oral tissues in conditions mimicking those in vivo. Results obtained in this study indicated a potential use of this system for studies on the mechanism of carcinogenesis at a cellular level comparable to in vivo carcinogenesis studies on oral tissues.