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Long‐term organ culture of hamster cheek pouch mucosa
Author(s) -
Mock David,
Main James H. P.
Publication year - 1976
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1976.tb01770.x
Subject(s) - cheek pouch , hamster , organ culture , oral mucosa , cheek , pouch , anatomy , explant culture , biology , pathology , medicine , microbiology and biotechnology , in vitro , biochemistry
. Explants of cheek pouch mucosa from 12‐ to 48‐hour‐old hamsters have been maintained in organ culture for up to 49 days with excellent preservation of tissue integrity and relationships. The technique uses gelatin sponge matrix in Leighton tubes with Eagle's Minimum Essential Medium (M.E.M.) supplemented with 10% calf serum and antibiotics.