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The effects of bacterial plaque extracts on organ cultures of fetal rat masticatory mucosa. I. Histochemistry
Author(s) -
Cobb Charles M.
Publication year - 1974
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1974.tb01695.x
Subject(s) - organ culture , tritrichomonas foetus , biology , fetus , acantholysis , pathology , acid phosphatase , oral mucosa , masticatory force , immunohistochemistry , lactate dehydrogenase , explant culture , microbiology and biotechnology , biochemistry , in vitro , anatomy , enzyme , immunology , medicine , pregnancy , genetics , orthodontics , antibody , autoantibody
. Cytotoxicity of pooled extracts from bacterial plaque was demonstrated by inoculating organ cultures of fetal rat masticatory mucosa. Advanced cytotoxic reactions were characterized by pyknosis, karyorrehexis, hyperkeratosis and possible acantholysis. Histochemical microscopy indicated possible alteration in Krebs cycle function and an increased anaerobic metabolism. Quantitative histochemistry demonstrated a statistically significant increase in total lactic dehydrogenase, glucose‐6‐phosphalc dehydrogenase and acid phosphatase in test explants when compared to controls. Test explants also exhibited a significant decrease in malic and isocitric dehydrogenase activities.