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Proteins in fluid from non‐keratinizing jaw cysts
Author(s) -
Skaug Nils
Publication year - 1973
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1973.tb01850.x
Subject(s) - fibrinogen , albumin , precipitin , immunoelectrophoresis , antiserum , globulin , blood proteins , chemistry , fibrin , macroglobulin , orosomucoid , microbiology and biotechnology , biology , biochemistry , glycoprotein , antibody , endocrinology , immunology
. Fluids from 32 non‐keratinizing jaw cysts were examined by polyacrylamide gel disc electrophoresis, and by immunoelectrophoresis and double diffusion in agar against rabbit anti‐human serum and rabbit antisera monospecific for six different plasma proteins. Albumin, α 1 ‐antitrypsin, α 1 ‐acid glycoprotein (orosomucoid), transferrin, IgG, IgA, and IgM were invariably recognized. Most cyst fluids also reacted with antiserum to α 1 ‐lipoprotein, α 2 ‐macroglobulin, β 1 C/β 1 A‐globulin (C3/C3c), and fibrinogen. Cyst fluid “fibrinogen” which showed somewhat slower electrophoretic migration than plasma fibrinogen, appeared as two precipitin bands or as one line with an apparently split cathodic end. Two cyst fluids exhibited one additional “fibrinogen” line located in the α 1 ‐globulin region. C3, which was not detected in all cyst fluids, displayed an electrophoretic mobility between that of β 1 C‐ and β 1 A‐globulin of serum. In two cases an even faster C3 subtraction appeared. Double diffusion gave a “reaction of identity” between albumin and the α‐ and β‐globulins of cyst fluid and autologous scrum. Cystic “fibrinogen” and fibrinogen of human plasma gave a “reaction of partial identity”. These findings indicate that albumin and the main α‐ and A‐globulins of fluid from non‐keratinizing jaw cysts are derived from plasma and that breakdown products of fibrinogen or fibrin are also present. High levels of IgG and IgA, but decreased amounts of α 2 ‐macroglobulin and C3 as compared to autologous serum are also suggested.