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Thymidine uptake and cell migration in cheek epithelium of zinc‐deficient rats
Author(s) -
Alvares O. F.,
Meyer J.
Publication year - 1973
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1973.tb01844.x
Subject(s) - weanling , epithelium , thymidine , endocrinology , medicine , cheek , biology , intraperitoneal injection , zinc , cell , cell growth , cell division , chemistry , anatomy , biochemistry , dna , organic chemistry , genetics
. Male weanling rats were fed a diet containing 1.3 mg zinc/kg diet and controls the same diet supplemented by 40 mg /zinc/kg diet. The rates of cell division and upward migration in the cheek epithelium were obtained from counts of labelled cells in animals sacrificed one hour and three days following intraperitoneal injection of 1 μCi tritiated thymidine/g body weight. A 3.4‐fold increase over the number of labelled cells in controls was observed one hour after thymidine administration and a 2.7‐fold increase three days later. Ratios of labelled cells at three days and one hour were 4.96 in controls and 3.99 in experimental animals. The percentage distribution of labelled cells in successive subdivisions of epithelium was identical in both groups. The number of labelled cells near the junction of granular and keratin layers was 2.9‐fold greater in the experimental animals. It is suggested that the zinc‐deficient cheek epithelium of the rat might be a useful model for the study of pathological conditions associated with increased rates of cell proliferation.