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Electron microscopic studies of organ cultures of tooth germs infected with polyoma virus
Author(s) -
Radden B. G.,
Main J. H. P.,
Ten Cate A. R.
Publication year - 1973
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1973.tb01690.x
Subject(s) - virus , biology , in vitro , tissue culture , epithelium , polyoma virus , cytoplasm , embryo , electron microscope , ultrastructure , mitosis , organ culture , virology , anatomy , microbiology and biotechnology , genetics , optics , physics
. Electron microscope studies were made of polyoma virus infected and uninfected organ cultures of tooth germs taken from 13‐ or 14‐day C 3 H mouse embryos. Cultures were examined at 18 days and at 38 or more days of in vitro age to include both morphologically transformed and untransformed odontogenic epithelium. Epithelial and mesenchymal cells were readily identified. Virus was seen in all infected cultures and in no controls. In nuclei the virus was found in both spherical and filamentous forms, while intracytoplasmic virus was only spherical in type. No virus was seen in nuclei of cells in mitosis. No difference was detected in the pattern of virus distribution between transformed and untransformed epithelial cells.