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Electron microscopy of enamel surface after reactions of apatite accretion
Author(s) -
Gonzalez M.,
Feagin F.
Publication year - 1973
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1973.tb01678.x
Subject(s) - apatite , scanning electron microscope , enamel paint , transmission electron microscopy , dissolution , fluorapatite , materials science , mineralogy , chemistry , calcium , nuclear chemistry , analytical chemistry (journal) , crystallography , metallurgy , chromatography , composite material , nanotechnology
. Images were obtained from intact and acid etched enamel surfaces (both before and alter growth of surface apatite) with electron diffraction, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Labial surfaces (intact) of bovine incisor teeth were slightly abraded and pumiced. They were then etched by a 14‐hour immersion in 2mM sodium acetate‐acetic acid buffer at pH 5.0; an average of 31 μ.Mol/cm 2 calcium and 24 μMol/cm 2 phosphate was dissolved from the surfaces. The SEM. and TEM showed loss of minerals to a depth of 10 μm. Dissolution was largely in central areas of enamel prisms, and prismatic structure in the etched surfaces was retained. Electron diffraction, SEM, and TEM showed the growth of crystalline apatites at the etched surfaces after 24 to 96 hours immersion in neutral pH solution (metastable) which contained 1.5 mM Ca +2 , 0.9 mM H 2 PO 4 /HPO 4 −2 , 0.05 mM F − , and 150 mM NaCl. Indirect analysis, at the loss of calcium and phosphate concentrations in the metastable solution after immersion periods, indicated deposition of approximately 24μMol/Cm 2 calcium and 14μMol/cm 2 phosphate on the etched surfaces. Results show induction of apatite crystal growth and recrystallization at enamel surfaces in otherwise stable solutions containing 0.05 mM F − .