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The acquired pellicle: Immunofluorescent demonstration of specific proteins
Author(s) -
ØRstavik Dag,
Kraus Frederick W.
Publication year - 1973
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1973.tb01675.x
Subject(s) - lysozyme , enamel paint , staining , chemistry , fixation (population genetics) , microbiology and biotechnology , pathology , biology , dentistry , biochemistry , medicine , gene
. Sterile polished slabs of bovine enamel, mounted in small acrylic cylinders, were held in the mouth for 2 hours. The acquired pellicles deposited on the enamel surfaces were examined in situ on the slabs or after transfer to microscope slides. Indirect immunofluorescent staining in situ afforded positive identification of IgA and lysozyme in pellicles. The preferred technique required acid separation of the pellicle from the enamel, transfer to a microscope slide, and fixation in cold ethanol. Histochemically, this pellicle appeared to he predominantly basic. Results of indirect immunofluorescent tracing indicated that IgA and lysozyme were regular constituents of the pellicle, amylase and IgG were less consistently detected, and albumin and fibrinogen were rarely encountered.