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Polymorphonuclear leukocyte collagenase: Localization in granules subjected to zonal centrifugation
Author(s) -
Robertson P. B.,
Ryel R. B.,
Fullmer H. M.,
Taylor R. E.,
Shyu K. W.
Publication year - 1972
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1972.tb01908.x
Subject(s) - granule (geology) , collagenase , polymorphonuclear leukocyte , centrifugation , alkaline phosphatase , granulocyte , chemistry , differential centrifugation , azurophilic granule , acid phosphatase , cell fractionation , biochemistry , sucrose , fractionation , peroxidase , sucrose gradient , microbial collagenase , microbiology and biotechnology , immunology , enzyme , biology , chromatography , myeloperoxidase , inflammation , paleontology , in vitro
. Polymorphonuclear leukocytes were obtained from rabbit peritoneal exudates. Granule preparations from these cells were subjected to zonal fractionation through a discontinuous sucrose gradient. Azurophilic and specific granules were enzymatically characterized by peroxidase and alkaline phosphatase, respectively. Collagenase was consistently associated with the alkaline phosphatase‐containing specific granules.