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The effect of physical treatment on the induction of heterotopic bone formation by demineralized dentin in guinea pigs
Author(s) -
Bang Gisle,
Johannessen Jan Vincents
Publication year - 1972
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1972.tb01661.x
Subject(s) - dentin , irradiation , transmission electron microscopy , chemistry , materials science , matrix (chemical analysis) , ultrastructure , anatomy , dentistry , biophysics , composite material , biology , medicine , physics , nuclear physics , nanotechnology
. A total of 388 allogenic demineralized guinea pig dentin samples, excluding 54 controls, were exposed to ionizing irradiation, ultrasonic vibration, heat, or storage at different temperatures. They were then subjected to amino acid analyses or transmission electron microscopy or implanted in the abdominal muscle of 74 male guinea pigs for four or five weeks. With increasing exposure to irradiation, heat or ultrasonic vibration, there was a decrease in the capacity of the implants to induce bone formation. This was most marked for the implants that had been subjected to 10.0 million rad or high temperature. Implants stored for more than approximately 8–12 weeks seemed to lose some of their bone‐inducing capacity, especially if stored at room temperature. Irradiation and heating resulted in profound changes in the dentin ultrastructure with swelling of the collagen fibrils and loss of cross‐striation. Specimens subjected to ultrasonic vibration showed some cleft formation in the dentin matrix but the collagen fibrils had retained cross‐striation. While irradiation caused a slight increase in acetic acid extractability of the dentin matrix, ultrasonic vibration and heating did not. It is suggested that the bone inducing capacity of dentin matrix is related lo a non‐collagenous protein.