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Baboon spermatology: basic assessment and reproducibility in olive baboons ( P apio anubis )
Author(s) -
Nyachieo Atunga,
Spiessens Carl,
Chai Daniel C.,
Kiulia Nicholas M.,
Mwenda Jason M.,
D'Hooghe Thomas M.
Publication year - 2012
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2012.00555.x
Subject(s) - baboon , electroejaculation , sperm , papio anubis , semen , biology , andrology , reproducibility , sperm motility , human fertilization , zoology , semen collection , anatomy , chemistry , medicine , endocrinology , artificial insemination , chromatography , pregnancy , genetics
Background Development of a reproducible baboon in vitro fertilization ( IVF ) system require optimized and reproducible sperm parameters. The objective of this study was to document basic spermatology values and investigate the reproducibility of these variables in the same baboons 1 or 3 months later in a larger number of baboons. Methods In this prospective study, sperm quality (semen volume, pH , concentration, motility, morphology and size) was evaluated in 27 sperm samples obtained from 9 baboons electroejaculated three times with a time interval of 1 month (between first and second sample collection) and 3 months (between second and third round sample collection). Results Baseline sperm values for semen volume (0.5 ± 0.3 ml), pH (7.5 ± 0.3), concentration (54.2 ± 19.3 million/ml), motility (67.3 ± 18.5%) and morphology (89.2 ± 4.8%) were similar to sperm samples obtained after 1 or 3 months ( P > 0.05). Head, midpiece and tail abnormalities were rarely observed (0–9%). Sperm dimensions were characterized by a tail length of 69.6 ± 13.9 μm, a head width of 2.41 ± 0.43 μm and a head length of 3.49 ± 0.6 μm. Conclusion Sperm quality was not affected by repeated electroejaculation with time intervals of 1 or 3 months, suggesting that the same baboon can participate multiple times in reproductive research.