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The conclusiveness of less‐invasive imaging techniques (computer tomography, X‐ray) with regard to their identification of bone diseases in a primate model ( Callithrix jacchus )
Author(s) -
Grohmann J.,
Taetzner S.,
Theuss T.,
Kuehnel F.,
Buchwald U.,
Einspanier A.
Publication year - 2012
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2011.00531.x
Subject(s) - callithrix , osteopontin , osteocalcin , cancellous bone , primate , immunohistochemistry , bone remodeling , pathology , bone mineral , anatomy , medicine , biology , alkaline phosphatase , osteoporosis , neuroscience , biochemistry , enzyme
Background Although common marmosets seem to be appropriate animal models to examine bone diseases, no data about the conclusiveness of less‐invasive techniques are available. Therefore, the aim was to combine different techniques to analyse changes in bone metabolism of common marmosets with bone diseases. Methods Five monkeys were examined by X‐ray, computer tomography (CT), histology and immunohistochemistry (IHC). Results Monkeys with lowest bone mineral density (BMD) showed increased bone marrow, decreased cancellous bone and decreased contrast in X‐ray. Highest alkaline phosphatase (AP)‐levels were detected in bones with low elastic modulus. Expression of osteopontin (OPN), osteocalcin (OC) and runt‐related transcriptions factor 2 (RUNX 2) was detected in bones with high modulus. No expression was present in bones with lower modulus. Collagen type I and V were found in every bone. Conclusions In conclusion, CT, X‐ray and AP are useful techniques to detect bone diseases in common marmosets. These observations could be confirmed by IHC.