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Development of a pigtail macaque model of sexually transmitted infection/HIV coinfection using Chlamydia trachomatis , Trichomonas vaginalis , and SHIV SF162P3
Author(s) -
Henning Tara,
Fakile Yetunde,
Phillips Christi,
Sweeney Elizabeth,
Mitchell James,
Patton Dorothy,
Sturdevant Gail,
Caldwell Harlan D.,
Evan Secor W.,
Papp John,
Michael Hendry R.,
McNicholl Janet,
Kersh Ellen
Publication year - 2011
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2011.00488.x
Subject(s) - chlamydia trachomatis , trichomonas vaginalis , coinfection , chlamydia , virology , sex organ , medicine , clearance , vaginitis , biology , immunology , gynecology , human immunodeficiency virus (hiv) , urology , genetics
Background Sexually transmitted infections (STIs) are associated with an increased risk of HIV infection. To model the interaction between STIs and HIV infection, we evaluated the capacity of the pigtail macaque model to sustain triple infection with Trichomonas vaginalis , Chlamydia trachomatis , and SHIV SF162P3 . Methods Seven SHIV SF162P3 ‐infected pigtail macaques were inoculated with T. vaginalis only (n = 2), C. trachomatis only (n = 1), both T. vaginalis and C. trachomatis (n = 2), or control media (no STI; n = 2). Infections were confirmed by culture and/or nucleic acid testing. Genital mucosa was visualized by colposcopy. Results Characteristic gynecologic signs were observed for both STIs, but not in control animals. Manifestations were most prominent at days 7–10 post‐infection. STIs persisted between 4 and 6 weeks and were cleared with antibiotics. Conclusions These pilot studies demonstrate the first successful STI‐SHIV triple infection of pigtail macaques, with clinical presentation of genital STI symptoms similar to those observed in humans.