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A non‐human primate model for analysis of safety, persistence, and function of adoptively transferred T cells
Author(s) -
Berger C.,
Berger M.,
Anderson D.,
Riddell S.R.
Publication year - 2011
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2010.00451.x
Subject(s) - biology , adoptive cell transfer , cd8 , cytotoxic t cell , t cell , cd19 , immunology , effector , cd28 , microbiology and biotechnology , antigen , in vitro , immune system , genetics
Background Adoptive immunotherapy with antigen‐specific effector T‐cell (T E ) clones is often limited by poor survival of the transferred cells. We describe here a Macaca nemestrina model for studying transfer of T‐cell immunity. Methods We derived, expanded, and genetically marked CMV‐specific CD8 + T E clones with surface markers expressed on B cells. T E cells were adoptively transferred, and toxicity, persistence, retention of introduced cell‐surface markers, and phenotype of the persisting T cells were evaluated. Results CD8 + T E clones were efficiently isolated from distinct memory precursors and gene‐marking with CD19 or CD20 permitted in vivo tracking by quantitative PCR. CD19 was a more stable surface marker for tracking cells in vivo and was used to re‐isolate cells for functional analysis. Clonally derived CD8 + T E cells differentiated in vivo to phenotypically and functionally heterogeneous memory T‐cell subsets. Conclusions These studies demonstrate the utility of Macaca nemestrina for establishing principles for T‐cell therapeutics applicable to humans.