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Frequency of the major histocompatibility complex Mamu‐A*01 allele in experimental rhesus macaques in China
Author(s) -
Wang Wei,
Cong Zhe,
Liu Xiuying,
Tong Wei,
Qiao Hongwei,
Jiang Hong,
Wei Qiang,
Qin Chuan
Publication year - 2010
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2010.00420.x
Subject(s) - allele , elispot , simian immunodeficiency virus , biology , rhesus macaque , major histocompatibility complex , virology , immunology , gene , genetics , antigen , virus , cd8
Background In Indian rhesus macaques, the major histocompatibility complex Mamu gene, especially the Mamu‐A*01 allele, plays an important role in simian immunodeficiency virus susceptibility and disease progression. The Mamu‐A*01 allele is one of the protective genes mostly being studied in simian acquired immunodeficiency syndrome. Methods PCR was used to amplify the Mamu‐A*01 allele in 130 Chinese‐origin rhesus macaques. Identification of the allele was then confirmed by sequencing and IFN‐γ ELISPOT assay. Results The Mamu‐A*01 allele was detected in 3.85% (5 of 130) of the experimental Chinese‐origin rhesus macaques. The sequence homology reached 99.1% in comparison with Indian rhesus macaques. A significantly large number of spots were observed in Mamu‐A*01 ‐positive monkeys when analyzed by ELISPOT with Gag181‐189 epitope stimulation. Conclusions Our study suggests that Mamu‐A*01 ‐positive Chinese‐origin rhesus monkeys are suitable for use in AIDS studies.