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Separate and combined effects of caffeine and dbcAMP on olive baboon ( Papio anubis ) sperm
Author(s) -
Nyachieo Atunga,
Spiessens Carl,
Chai Daniel C.,
Kiulia Nicholas M.,
Mwenda Jason M.,
D’Hooghe Thomas M.
Publication year - 2010
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2010.00401.x
Subject(s) - baboon , capacitation , sperm , sperm motility , andrology , human fertilization , caffeine , papio anubis , incubation , biology , zona pellucida , motility , endocrinology , oocyte , embryo , anatomy , medicine , biochemistry , genetics , microbiology and biotechnology
Abstract Background Improvement of baboon sperm capacitation is necessary for achieving high in vitro fertilization (IVF) rates in baboons. In this study, we evaluated separate and combined effects of caffeine and dbcAMP on baboon sperm capacitation. Methods Sixteen male baboons (n = 16) were electroejaculated. Each sperm sample was divided into two aliquots: one for chemical activation and the other untreated control. Group 1: dbcAMP (n = 6); Group 2: caffeine (n = 6) and Group 3: combination of caffeine and dbcAMP (n = 4). In each aliquot, sperm motility after 30 minutes of incubation was evaluated as well as zona pellucida (ZP) binding ability after overnight incubation with 4–5 ZP from unfertilized human oocytes. Results Sperm motility and ZP binding ability in all chemically activated groups increased significantly as compared to their respective controls ( P < 0.05). Conclusion Combined and separate effects of caffeine and dbcAMP increases baboon sperm motility and ZP binding ability and may improve baboon IVF.