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Surface phenotype and rapid quantification of blood dendritic cell subsets in the rhesus macaque
Author(s) -
Brown Kevin N.,
BarrattBoyes Simon M.
Publication year - 2009
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2009.00353.x
Subject(s) - rhesus macaque , cd11c , flow cytometry , dendritic cell , whole blood , biology , immunology , interleukin 3 receptor , cd16 , antibody , cd8 , plasmacytoid dendritic cell , macaque , blood cell , immunophenotyping , phenotype , microbiology and biotechnology , antigen , cd3 , genetics , gene , neuroscience
Background The study of dendritic cell (DC) biology in the rhesus macaque is becoming increasingly important but is limited by incomplete characterization and the lack of a rapid assay to quantify cells. Methods We characterized the surface phenotype of myeloid (mDC) and plasmacytoid DC (pDC) subsets in healthy rhesus macaque blood and developed a flow cytometry‐based assay for absolute DC determinations. Results Rhesus CD11c + mDC were CD16 + CD11b + CD56 lo CD8 − CD1c − whereas CD123 + pDC lacked expression of these markers. Precise DC determinations were performed using a rapid two‐step assay combining the analysis of whole blood and peripheral blood leukocytes (PBL). Conclusions Antibodies to CD11b, CD56 and CD16 must be omitted from the lineage antibody cocktail to prevent inadvertent gating‐out of DC when analyzing rhesus blood. The combined whole‐blood/PBL quantification assay will be invaluable for the rapid and repeated monitoring of blood DC counts in this species.