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Molecular cloning, gene expression, and tissue distribution of adiponectin and its receptors in the Japanese monkey, Macaca fuscata
Author(s) -
Kang KyungHwa,
Higashino Atsunori,
Kim HeuiSoo,
Lee YongTae,
Kageyama Takashi
Publication year - 2009
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2008.00298.x
Subject(s) - adiponectin , adiponectin receptor 1 , adipokine , biology , adipose tissue , receptor , medicine , endocrinology , gene , messenger rna , adipocyte , insulin resistance , insulin , genetics
Background Adiponectin is an adipocyte‐derived hormone that affects regulation of metabolic syndrome such as insulin resistance, type‐2 diabetes, and obesity. It functions via seven transmembrane domain receptors [i.e., adiponectin receptors 1 (AdipoR1) and 2 (AdipoR2)] that have been scarcely investigated in non‐human primates. Methods Molecular cloning of cDNAs for adiponectin, AdipoR1, and AdipoR2 that included the whole protein‐coding region in the Japanese monkey, Macaca fuscata , was carried out. Tissue‐specific expression of respective genes was analyzed with Northern blot hybridization. Results The essential Cys36 and four lysine residues in adiponectin, and transmembrane‐spanning domains in AdipoR1 and AdipoR2 appear well conserved. While adiponectin mRNA is expressed only in adipose tissues, AdipoR1 mRNA was found to be expressed in various tissues including the brain. Conclusions These results significantly add to the understanding of the molecular basis of obesity‐related adipokines and their receptors in non‐human primates.