Premium
Development, validation and application of a chemiluminescent immunoassay for the measurement of circulating chorionic gonadotropin levels in the laboratory macaque
Author(s) -
Lohstroh Pete,
Laughlin Lisa,
Gee Nancy,
Lasley Bill
Publication year - 2007
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2006.00189.x
Subject(s) - human chorionic gonadotropin , autoanalyzer , chemiluminescence , gonadotropin , immunoassay , chemiluminescent immunoassay , pregnancy test , medicine , biology , immunology , chemistry , chromatography , pregnancy , endocrinology , hormone , antibody , genetics
Background Methods for the measurement of chorionic gonadotropin for pregnancy detection in laboratory macaques chorionic gonadotropin (mCG) have been limited by the paucity of specific reagents. Current assays use reagents developed for human chorionic gonadotropin (hCG) assays and have been limited to radio‐ or enzyme‐immuno assays. Methods This report describes an automated assay for mCG using reagents for hCG detection that has been adapted to the automated chemiluminescence platform of the Bayer ACS‐180 autoanalyzer. Results This automated assay performs similarly to previous assays in terms of sensitivity and specificity but provides additional speed, reliability, and more easily accessible reagents compared with previous formats. Conclusions When implemented broadly, this assay could provide standardization of mCG measurements for both research and colony management purposes.