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Polyvalent DNA prime and envelope protein boost HIV‐1 vaccine elicits humoral and cellular responses and controls plasma viremia in rhesus macaques following rectal challenge with an R5 SHIV isolate
Author(s) -
Pal Ranajit,
Wang Shixia,
Kalyanaraman V.S.,
Nair B.C.,
Whitney Stephen,
Keen Timothy,
Hocker Lindsey,
Hudacik Lauren,
Rose Nicolas,
Cristillo Anthony,
Mboudjeka Innocent,
Shen Siyuan,
WuChou TeHui,
Montefiori David,
Mascola John,
Lu Shan,
Markham Phillip
Publication year - 2005
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2005.00120.x
Subject(s) - viremia , virology , biology , heterologous , immune system , antibody , priming (agriculture) , dna vaccination , homologous chromosome , immunology , gene , immunization , genetics , germination , botany
Immunization of macaques with multivalent DNA encoding gp120 genes from HIV‐1 subtypes A, B, C and E and a gag gene followed by boosting with homologous gp120 proteins elicited strong anti‐gp120 antibodies capable of neutralizing homologous and to a lesser degree heterologous HIV‐1 isolates. Both Env‐ and Gag‐specific cell mediated immune (CMI) responses were detected in the immunized animals. Following rectal challenge with an SHIV isolate encoding HIV‐1 Ba‐L env , plasma viremia in the infected immunized animals was significantly lower than that observed in the naïve animals. Further, one of six immunized animals was completely protected whereas all six naïve animals were infected. These results demonstrate that a vaccine based on priming with a polyvalent DNA vaccine from multiple HIV‐1 subtypes followed by boosting with homologous Env proteins elicits anti‐HIV‐1 immune responses capable of controlling rectal transmission of SHIV Ba‐L .