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Molecular cloning and in vitro evaluation of an infectious simian‐human immunodeficiency virus containing env of a primary Chinese HIV‐1 subtype C isolate
Author(s) -
Wu YingYun,
Hong KunXue,
Chenine AgnèsLaurence,
Whitney James B.,
Chen QiMin,
Geng YunQi,
Ruprecht Ruth M.,
Shao YiMing
Publication year - 2005
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2005.00098.x
Subject(s) - virology , simian immunodeficiency virus , biology , simian , virus , peripheral blood mononuclear cell , human immunodeficiency virus (hiv) , in vitro , genetics
Human immunodeficiency virus (HIV) clade C is the most prevalent subtype and accounts for approximately 50% of all HIV infections worldwide. In China, the prevalent HIV strains are B′/C subtypes, in which the envelope belongs to subtype C. To evaluate potential AIDS vaccines targeting Chinese viral strains in non‐human primate models, we constructed an infectious simian‐human immunodeficiency virus (SHIV) that expresses most of the envelope of a primary HIV strain, which was isolated from a HIV‐positive intravenous drug user from XinJiang province in China. The resulting chimeric SHIV‐XJ02170 was infectious in human, rhesus monkey and cynomolgus monkey peripheral blood mononuclear cells (PBMC) and used CCR5 exclusively as coreceptor.