Premium
Cell‐free systems for capsid assembly of primate lentiviruses from three different lineages
Author(s) -
Dooher Julia E.,
Lingappa Jaisri R.
Publication year - 2004
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2004.00075.x
Subject(s) - capsid , primate , biology , group specific antigen , lentivirus , lineage (genetic) , simian immunodeficiency virus , microbiology and biotechnology , virology , human immunodeficiency virus (hiv) , virus , genetics , gene , neuroscience , viral disease
We recently demonstrated that capsids from three main primate lentiviral lineages appear to form via a pathway of assembly intermediates in primate cells. Retroviral capsid assembly intermediates were initially identified and characterized using a cell‐free system for assembly of immature HIV‐1 capsids. Because cell‐free capsid assembly systems are useful tools, we are interested in developing such systems for other primate lentiviruses besides HIV‐1. Here we extend previous cell‐free studies by showing that Gag proteins of HIV‐2, from a second primate lentiviral lineage, progress from early intermediates to late intermediates and completed capsids over time. Additionally, we demonstrate that Gag proteins of SIVagm, from a third primate lentiviral lineage, associate with the cellular factor HP68 and complete assembly in this system. Therefore, cell‐free systems reproduce assembly of Gag from three main primate lentiviral lineages, and can be used to compare mechanistic features of capsid assembly of genetically divergent primate lentiviruses.